Journal: FASEB BioAdvances
Article Title: Preference and detrimental effects of high fat, sugar, and salt diet in wild‐caught Drosophila simulans are reversed by flight exercise
doi: 10.1096/fba.2020-00079
Figure Lengend Snippet: Effects of different diets on activity, survival, and development. (A): Total activity was recorded in a group of 5 flies in narrow vials over 4 days in LAM25H TriKinetics activity monitor (n = 9 replicates, 5 flies per replicate). Y‐axis ‐activity counts. Two‐way ANOVA. Tukey's multiple comparisons test. Significant differences between different diets and the CD: †‐ versus Control females ( p < 0.0001), # ‐ versus Control males ( p < 0.0001). Significant difference between different diets and WD: ∅ ‐ versus WD females ( p < 0.01–0.0001), ∇ ‐ versus WD males ( p < 0.05). (B): Representative graph of circadian activity pattern of females and males maintained on a 12‐h LD cycle (lights on at 9:00am) over 4 days. Note a decrease in activity on the WD during daytime and increase at nighttime. Locomotor activity is expressed as the mean number of beam breaks per hour. (C): Total activity during night (9 pm ‐ 9 am) on different diets. Two‐way ANOVA, Tukey's multiple comparisons test: △‐ significant difference between WD females and other female groups ( p < 0.0001), $‐ significant difference between WD males and SD males ( p < 0.05). (D): Cumulative survival over 14 days. Results are averages of nine replicates of 5 flies per vial. One‐way ANOVA. *‐ Significant difference between WD females and other female groups ( p < 0.01–0.0001). (E, F): Survival of females on different diets (E). Two‐way ANOVA. a‐ significant difference between WD females and CD females ( p < 0.0001). Survival of males on different diets (F). The number of flies that survived was determined for each cohort once per day. Two‐way ANOVA. *‐ significant difference between WD males and CD males ( p < 0.05 – 0.0001). (G): Total number of pupae (mean ±s.e.m.) emerging from larvae sired by different fathers (CF, EF, WF, WEF). Two‐way ANOVA. b ‐ significant difference between WD and CD groups ( p < 0.0001). (H): Cumulative number of eclosed adult flies (mean ±s.e.m.) sired by different fathers. Two‐way ANOVA. Tukey's multiple comparisons test: b ‐ significant difference between WD and CD groups ( p < 0.0001). Error bars represent SEM. All experiments were repeated three times.
Article Snippet: Fly locomotor activity was measured in TriKinetics LAM25H locomotor activity apparatus.
Techniques: Activity Assay, Control
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